lab 2 bio copy

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Wilmington University *

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150

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Biology

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Apr 29, 2024

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docx

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4

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Page 1 Page 1 Bio 150- Laboratory Report Human Anatomy & Physiology I DCCC Buffers Lab Report # 3 - pH & Buffers I. A. Data Tables 1. List two things that should be included in every data table. A. Number and title for graph B. Label the dependent and independent variables 2. Define independent variable. Independent variable is the factor that is being controlled or modified to determine how it affects the dependent variable I. B. Graphs 1. List two things that should be included in every graph. A. Units of measurement B. A title 2. Which variable is usually found on the X axis? Independent variable II. Introduction to pH Define the following terms: 1. acid: ph 0-6 is a proton donor ;h+ is a chemical that releases protons into a solution 2. base: ph 8-14 is a proton acceptor , binds protons from solution 3. salt: is neutral which is obtained by neutralization of hydrochloric acid and sodium hydroxide, Neutralization of any strong acid with a strong base always gives a neutral salt. 4. buffer: buffers are Solutions that prevent large changes in pH levels in the solution. . 5. pH scale: measure of the concentration of hydrogen ions (H+) in a solution III. Equipment 1. Name three pieces of equipment that are shown in the Buffers Equipment Video. A. Ph meter Last updated: 07/2021
Page 2 Page 2 Bio 150- Laboratory Report Human Anatomy & Physiology I DCCC Buffers B. Hot plate C. Beakers IV. pH of Strong Acids, Weak Acids, Strong Bases, and Weak Bases Use the Strong & Weak Acid-Base pH Demo video to answer the following questions: 1. What substance was used to observe the pH of a strong acid? Sulfuric acid 2. What was the pH of the weak acid? 4.0 3. What was the pH of the strong base? 14.0 4. What substance was used to observe the pH of a weak base? Sodium acetate V. A. Introduction 1. After 1 drop of sodium hydroxide was added to each of the test tubes, how did the test tubes containing the buffer differ from the test tube containing water? With the first and second test tubes with the buffers there was no change and in the H2O was a change and it immediately turned purple so it’s not a buffered solution 2. How many drops of sodium hydroxide solution did it take to reach the buffer’s capacity in test tube number 2 (with the 0.01M sodium acetate buffer)? 25 drops V. B. Experiment 1 1. Construct a data table for Experiment 1. Use a ruler to construct the table. Include a 0 drop (no HCl added to the beaker) pH reading in the table. Follow the directions in part I of the lab manual to construct the table. 2. Using the graph paper below, construct a graph that contains the data from your table in question 1 (above). Follow the directions in part I of the lab manual to construct the graph. Last updated: 07/2021
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