ANTH 270 Lab 3 Worksheet (1)
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Anthropology 270
Lab 3: Cladistics and Evolutionary Relationships
Name: Diego Ryan Section Time: Thursday at 4PM
Part 1: Introduction (read this before coming to labs)
Humans are very good at recognizing patterns, which makes our species particularly fond of categorization and organization. To make sense of the natural world humans have long attempted to organize, catalog and classify Earth's “endless forms.” One of these famous organizers was the Swedish botanist and zoologist, Carolus Linnaeus. Known today as the Father of Modern
Taxonomy,
Linnaeus formalized the Latin-based naming system (
binomial nomenclature
) for categorizing plants, animals, and “minerals.” Although many of Linneaus’ original classifications have been reordered thanks to advancements in technology (e.g., we now know that manatees and whales are mammals, not fish), he was the first to correctly classify humans within the taxonomic order Primates, and was the first to classify bats as mammals (not birds).
Another approach to biological classification,
cladistics
, focuses on grouping organisms based on shared
derived traits
(i.e., novel evolutionary traits that are unique to a particular species and all its descendants) in order to infer their "true" evolutionary relationships. These groupings are often visualized as a
cladogram,
or
tree
. Trees generally follow the
Law of Parsimony
, a scientific principle that states that the simplest explanation is the most likely, and therefore best, explanation. For example, if all members of a group were blue, the simplest explanation would be that they are blue because their common ancestor was also blue. In this case, the blue trait evolved
once
in the common ancestor and was passed on to all of its' descendants. To say that the blue trait evolved separately in each lineage is more complex and, therefore, less parsimonious.
When organisms share a trait that was inherited from a common ancestor (e.g., the blue trait from the example above), the trait is called a
homology
. For example, although
the arms of bats, humans, whales, and cats
look very different on the outside (reflecting differences in locomotion), the bones on the
inside
are homologous. This is because they inherited these bones (i.e., humerus, radius, ulna, carpals, metacarpals and phalanges) from a common mammalian ancestor who possessed them. Trees made from homologous traits are called
phylogenies
. Phylogenies reflect
evolutionary relationships
because they are based on inheritance and common descent.
It is important to note that not all trees show the “true” evolutionary history of organisms. Just because two species
look
alike or possess the same trait does not mean that they inherited that trait from a common ancestor. For example,
both bats and birds can fly and have wings
, but they did not inherit wings from their common ancestor. The bat-bird common ancestor did not fly or possess wings. Similarly, whales and seals are both aquatic mammals that possess flippers, but they didn’t inherit flippers from their common ancestor (their common ancestor was a terrestrial mammal). When organisms share a trait, but did not inherit it from a common ancestor (e.g., bat and bird wings), the trait is called an
analogy
. Analogous traits can arise through
reversals
(i.e., when a previously gained trait is lost) or
convergent evolution
, which occurs when species occupy the same ecological niche or live in similar environments (e.g., both whales and seals live in the ocean). Because analogies
do not
reflect evolutionary relationships, these traits should not be used to build trees!
1
In-Class Lab Activity
During lab today you will watch
Tree Diagrams
and discuss how to build and interpret phylogenetic
trees. Once we are familiar with making trees, we will put those skills to the test! For this lab activity we will be making two different types of trees; a cladogram based on phenotypic data (Part 1), and a phylogeny based on genetic data (Part 2).
Part 2: Building a Tree from Phenotypic Data
First, take a look at the 7 animals in the Lab section of our Week 3 module. The table below shows
the presence/absence of 9 traits across the 7 mammals. Using only these traits, make the
most parsimonious
tree. You’ll need a piece of scratch paper and pencil. Hold onto your tree for the next step, but you will not turn in the tree.
Your tree must abide by the following rules:
1.
Your tree must be
dichotomous
(i.e., each branch of the tree must be split in two, usually with the organisms on one branch
having
the trait and the organisms on the other branch
not having
the trait),
2.
You must put
labeled
tick-marks
on the branches of your tree showing which traits you used to make your groupings (remember that
all
organisms past the tick-mark
have
this trait), and
3.
Each branch of your tree must ultimately end in one species (i.e., each of the 7 mammals will end up on its own branch at the tip of the tree).
Table 1. Presence or absence of 9 traits (rows) across the 7 mammals (columns)
Trait
Echidna
Pangolin
Slot
h
Armadillo
Porcupine
Lio
n
Human
Produces milk
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Live birth
No
Yes
Yes
Yes
Yes
Yes
Yes
Teeth
No
No
Yes
Yes
Yes
Yes
Yes
Fused ischium/sacrum
No
No
Yes
Yes
No
No
No
4-chambered stomach
No
No
Yes
No
No
No
No
Tooth enamel
No
No
No
No
Yes
Yes
Yes
Continuously growing
incisors
No
No
No
No
Yes
No
No
Increased sociality
No
No
No
No
No
Yes
Yes
Opposable thumb
No
No
No
No
No
No
Yes
2
Part 2: Building a Phylogeny from Genetic Data
Sometimes different datasets yield different results. This is especially true when making trees, as phenotypic data and genetic data can sometimes tell different evolutionary stories based on the specific combination of traits or genetic loci used. Here we will incorporate the genetic data from these 7 mammals to build a
phylogeny
showing their evolutionary relationships. We will then compare the trees that we made using the different datasets.
To help you visualize this genetic data (in the form of nucleotide sequences), we have provided a table showing the first 15 nucleotide bases of a gene from each species below:
Table 2 DNA sequences (rows) for 7 Mammals
Echidna
C
A
A
T
T
C
T
G
G
C
C
G
T
C
C
Pangolin
T
C
A
T
A
G
T
C
G
T
A
T
T
A
G
Sloth
C
T
A
T
G
G
T
C
G
T
A
G
T
C
T
Armadillo
C
T
A
T
G
G
T
C
G
T
A
G
T
C
T
Porcupine
T
G
A
T
C
G
T
C
G
T
A
C
T
C
A
Lion
T
C
A
T
A
G
T
C
G
T
A
T
T
A
G
Human
T
G
A
T
C
G
T
C
G
T
A
C
T
C
A
You will notice that the colorful nucleotide sequences from the table above have been converted to
FASTA format
in the box below. FASTA format is a popular format for displaying sequence data and is required by many phylogenetic programs like the one we will use today.
Table 3. Multiple Species Alignment for 7 Mammals.
>Echidna
CAATTCTGGCCGTCC
>Pangolin
TCATAGTCGTATTAG
>Sloth
CTATGGTCGTAGTCT
>Armadillo
CTATGGTCGTAGTCT
>Porcupine
TGATCGTCGTACTCA
>Lion
TCATAGTCGTATTAG
>Human
TGATCGTCGTACTCA
3
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